Posters
Please find below posters that are based on High Resolution Melting.
Grundy et al, "Calibration Improves Methylation Sensitive High Resolution Melting Analysis" presented at the ESHG 1st-3rd June 2008 Download PDF...
Churchill Hospital, Dept of Medical Genetics: "Fast service for familial Hypertrophic and Dilated Cardiomyopathy using High Resolution Melt Curve Analysis and the LightScanner Download PDF...
Tp53 Mutation Screening using High Resolution Melting on the HR1 Download PDF...
Detection of Somatic Tumors Mutations by High Resolution Melting Download PDF...
Internal Calibrators for High Resolution Melting Download PDF...
Digital PCR and High Resolution Melting Download PDF...
Published References
Please find below a selection of published references using High Resolution Melting Analysis. More can be found on the Pub Med web site.
1) Gundry CN, Vandersteen JG, Reed GH, Pryor RJ, Chen J, Wittwer CT.: Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes. Clin Chem. 2003 Mar;49(3):396-406.
2) Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ.: High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem. 2003 Jun;49(6 Pt 1):853-60.
3) Montgomery J, Wittwer CT, Kent JO, Zhou L. :Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis. Clin Chem. 2007 Nov;53(11):1891-8. Epub 2007 Sep 21.
4) Zhou L, Myers AN, Vandersteen JG, Wang L, Wittwer CT.: Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye. Clin Chem. 2004 Aug;50(8):1328-35. Epub 2004 May 27.
5) Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT, Voelkerding KV.: Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes. Clin Chem. 2006 Mar;52(3):494-503. Epub 2006 Jan 19.
6) Liew M, Pryor R, Palais R, Meadows C, Erali M, Lyon E, Wittwer C.: Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem. 2004 Jul;50(7):1156-64.
7) Zhou L, Wang L, Palais R, Pryor R, Wittwer CT.: High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution. Clin Chem. 2005 Oct;51(10):1770-7.
8) Herrmann MG, Durtschi JD, Wittwer CT, Voelkerding KV.: Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping. Clin Chem. 2007 Aug;53(8):1544-8. Epub 2007 Jun 7.
9) Eggerding FA.: A one-step coupled amplification and oligonucleotide ligation procedure for multiplex genetic typing. PCR Methods Appl. 1995 Jun;4(6):337-45.
10) Graziano C, Giorgi M, Malentacchi C, Mattiuz PL, Porfirio B.: Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes. BMC Med Genet. 2005 Oct 4;6:36.
11) Liew M, Seipp M, Durtschi J, Margraf RL, Dames S, Erali M, Voelkerding K, Wittwer C.: Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting. Am J Clin Pathol. 2007 Mar;127(3):341-8.
12) Bohling SD, King TC, Wittwer CT, Elenitoba-Johnson KS. :Rapid simultaneous amplification and detection of the MBR/JH chromosomal translocation by fluorescence melting curve analysis. Am J Pathol. 1999 Jan;154(1):97-103.
13) Seipp MT, Pattison D, Durtschi JD, Jama M. Voelkerding KV, Wittwer CT.: Quadruplex Genotyping of F5, F2, and MTHFR Variants in a Single Closed Tube by High-Resolution Amplicon Melting. Clin Chem. 2008 Jan;54(1):108-15.
14) Sundberg SO, Wittwer CT, Greer J, Pryor RJ, Elenitoba-Johnson O, Gale BK.: Solution-phase DNA mutation scanning and SNP genotyping by nanoliter melting analysis. Biomed Microdevices. 2007 Apr;9(2):159-66.
15) Montgomery J, Wittwer CT, Palais R, Zhou L.: Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis. Nat Protoc. 2007;2(1):59-66.
16) Chou LS, Lyon E, Wittwer CT: A comparison of high-resolution melting analysis with denaturing high-performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model. Am J Clin Pathol. 2005 Sep;124(3):330-8.
17) Hill CE, Duncan A, Wirth D, Nolte FS: Detection and identification of cytochrome P-450 2C9 alleles *1, *2, and *3 by high-resolution melting curve analysis of PCR amplicons. Am J Clin Pathol. 2006 Apr;125(4):584-91.
18) Seipp MT, Durtschi JD, Liew MA, Willams J, Damjanovich K, Pont-Kingdon G, Lyon E, Voelkerding KV, Wittwer CT. :Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting. J Mol Diagn. 2007 Jul;9(3):284-9.
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