Assay For Detection of Inflammation and Tissue Injury
Neutrophil CD64 expression is rapidly increased both in vitro and in vivo within hours by mediators of inflammation, such as interferon-gamma and G-CSF. The same change is observed in response to documented infection or tissue injury, thus indicating that the measurement of neutrophil CD64 expression correlates with the presence of such conditions in humans.

The Leuko64 kit is composed of a mixture of three monoclonal antibodies with specificities to CD64 (both FITC conjugated) and CD163 (phycoerythrin conjugated), a concentrated lysing reagent, and a fluorescent bead suspension. The beads are used for instrument calibration and standardization of the leukocyte CD64 and CD163 expression in human blood. The Leuko64 assay is intended for use in the measurement of leukocyte neutrophil CD64 levels, which increase in response to tissue injury.

Automated software for flow cytometric listmode data analysis using iterative cluster finding algorithms is included in the assay kit. This software was co-developed with Verity Software House (www.vsh.com) and provides for objective data analysis and result.

Key Features Requires only 50 µL of blood and under one hour of hands-on time High correlation with presence of infection · sensitivity 90.5% · specificity 96.3% · positive predictive value 95.0% · negative predictive value 92.9% Standardized assay with internal calibrator and controls Automated user-friendly software allows for excellent reproducibility (CV <5.0%)