The Coating Buffer for ELISA, EIA, RIA, protein array and immuno PCR.

Coating Buffer is made for adsorptive immobilisation of proteins and antibodies on plastic surfaces (for example microtiterplates) or other protein binding surfaces. Depending on surface as well as on proteins or antibodies the required incubation times can differ. Consequently any user should optimise its own incubation.

Buffer optionally based on PBS (pH 7,4) or carbonate (pH 9,6). For some proteins or antibodies Coating Buffer pH 7,4 is better , for others Coating Buffer pH 9,6 is better for immobilisation. The pH-value can have an influence on the steric structure of proteins or antibodies, thus having an effect on immobilisation.

For an optimised procedure of a newly developed immunoassay we strongly recommend testing both Coating Buffers in comparison.

Details 10x stock solution Dilute with water before using! For research use only, not for diagnostic use Available Packaging Size Coating Buffer pH 7,4 (based on PBS) · 125 ml order number 120125 Coating Buffer pH 9,6 (based on carbonate) · 125 ml order number 121125