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Ancillary Products
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IQ Lyse
IQ Lyse(1) is a fIow cytometry Iysing solution intended for lysing of erythrocytes. IQ Lyse is a ready to use product, which can be stored at room temperature till the given expiration date. For optimal results IQ Lyse should be used following the complete procedure as indicated below (standard procedure). A shortened method can be applied as well (also described as ‘lyse-no-wash’ method). Blood samples processed with IQ Lyse will appear in the reagent tube as a reddish cell suspension after lysing. The red color does not influence staining results and subsequent fIow cytometry analysis.
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IQ BrightLyse Red Cell Lysing reagent
Summary
Brightlyse is an erythrocyte (RBC) lysing solution for whole blood
Intended Use
Brightlyse is a RBC lysing solution to be used in a lyse-no-wash procedure for flow cytometric enumeration of antigenic markers.
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IQ Starfiqs Is a fixation and permeabilization solution intended for preparation of blood leukocytes for flow cytometry analysis of intracellular antigens. IQ Starfiqs™ is a ready to use product, and is suitable for use with whole blood or isolated leucocytes. It is composed of two reagents used sequentially and they have been developed to ensure optimum performance in flow cytometry.
Fixation and permeabilization with IQ Starfiqs™ enables the detection of intracellular cytokines, intracellular antigens such as, CyCD3, CyCD22, TdT and MPO, and has also been used successfully to fix and permeabilize cells in the TUNEL assay for apoptosis. In addition, IQ Starfiqs™ allows the simultaneous detection of cell surface antigens.
For optimal intracellular immuno-staining and lysing of erythrocytes in the case of whole blood protocols, IQ Starfiqs™ should be used following the procedures described. The protocol will depend on the type of antigen and whether whole blood or isolated lymphocytes are being used. It is important to use both reagents and not to mix with other products. IQ Starfiqs™ is provided as a ready to use product, to minimize hands on time and the easy handling of samples.
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